| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1398-1 | ||||
Resumo:Drought is a major challenge faced by agriculture, not only in Brazil, but also in various regions of the world. Given this scenario, it is important to search for solutions that can minimize the effects of drought on different crops. Among the alternatives, the use of biological inputs based on plant growth-promoting bacteria (PGPB) is a sustainable and low-cost alternative. This work aimed to isolate, characterize and identify bacteria present in soils collected from different Brazilian ecosystems, with emphasis on tolerance to drought and saline stresses. A total of 700 thermotolerant strains were isolated from 297 soil samples, especially from the Brazilian caatinga region, and selected based on macromorphological characteristics. All strains were evaluated for their ability to grow in solid culture medium (Luria Bertani – LB) containing high concentrations of sodium chloride or sorbitol in order to simulate conditions of low water availability in the soil. A total of 354 strains grew in medium containing 10% NaCl (w/v), while 151 strains grew in culture medium containing a high concentration of sorbitol (520 g/L). Of these, 89 strains were able to grow both in media containing NaCl and sorbitol. Initially, the repetitive extragenic palindromic PCR (REP-PCR) and the enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) were used for the typing of the species. Later, the strains with different REP-PCR and ERIC-PCR banding profiles were sequenced and identified as Priestia megaterium, P. aryabhattai, Bacillus subtilis, B. licheniformes, B. altitudinis and B. safensis. PGPB have the potential to increase plant health under abiotic soil stresses associated with global climate change (viz., drought and salinity). The application of stress-resistant PGPB may not only help in the combating the effects of abiotic stressors and increase yield, but also lead to mitigation of climate change. Palavras-chave: Bacillus, Bacteria, Drought, Priestia, Salinity Agência de fomento:Embrapa, CNPq, Finep, Fapemig | |||||